Environmental detection of SARS-CoV-2 in hospital rooms in different wards of an University Hospital

Source avec lien : Journal of Hospital Infection, (Prépublication). 10.1016/j.jhin.2022.07.029

L’excrétion virale pendant la période Covid-19 ne semble pas liée à la présence de symptômes, à l’âge du patient et au seuil de cycle de valeur faible du test du patient. Cette étude confirme la preuve de l’excrétion environnementale du SRAS-CoV-2 jusqu’à au moins 7 jours après l’apparition des symptômes du patient. Elle souligne la nécessité d’un respect strict des précautions à prendre en cas de contact, de l’hygiène des mains, de l’utilisation correcte des équipements de protection individuelle et de la désinfection des chambres pour les soins de routine des patients atteints du syndrome Covid-19.

Background Transmission of SARS-CoV-2 can occur through direct, indirect, or close contact with infected people. However, the extent of environmental contamination is unknown. The nature of the relation between patients’ symptoms and SARS-CoV-2 environmental shedding remains unclear. The aim of this study was to assess the relationship between patient covid-19 status and environmental contamination. Methods Between May to November 2020, environmental swabs were taken before and after room disinfection at day 7 after symptom onset in a cohort of patients clinically or biologically diagnosed positive for Covid-19. Twelve surfaces per room were collected in 13 rooms. Sample analysis was performed by RT-PCR for SARS-CoV-2 detection (SARS-CoV-2 R-Gene® (Biomérieux)). Clinical data (day of illness, symptoms, RT-PCR results) was collected from the clinical software. Results Five medical units were included in the study. Of 156 samples collected in 13 rooms, 5 rooms (38.5%) presented 11 SARS-CoV-2 positive samples. These positive samples were detected on 8 different surfaces. There was no association between detection of SARS-CoV-2 and patient’s age (p= 1) or patient’s symptoms (p= 0.3). Conclusion Viral shedding during Covid-19 appears unrelated to the presence of symptoms, patient’s age, and low value cycle threshold of patient’s test. This study supports the evidence for the environmental shedding of SARS-CoV-2 until at least 7 days after patient’s symptoms onset. It emphasizes the need for strict compliance with contact precaution, hand hygiene, the correct use of personal protective equipment and a room disinfection for the routine care of Covid-19 patients.

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