Source avec lien : Indoor Air, 32(11). 10.1111/ina.13161
Nous avons développé et validé un nouveau dispositif AMPAS (Automated Multiplate Passive Air Sampling). Il permet de collecter des séquences d’échantillons de dépôt passif sur une période de temps contrôlée sans intervention humaine. L’AMPAS a été utilisé avec l’échantillonnage d’air pour mesurer l’effet du taux de ventilation et de l’emplacement spatial sur le taux de dépôt de Staphylococcus aureus aérosolisé dans une chambre.
Abstract Hospital-acquired infections (HAIs) are a global challenge incurring mortalities and high treatment costs. The environment plays an important role in transmission due to contaminated air and surfaces. This includes microorganisms’ deposition from the air onto surfaces. Quantifying the deposition rate of microorganisms enables understanding surface contamination and can inform strategies to mitigate the infection risk. We developed and validated a novel Automated Multiplate Passive Air Sampling (AMPAS) device. This enables sequences of passive deposition samples to be collected over a controlled time period without human intervention. AMPAS was used with air sampling to measure the effect of ventilation rate and spatial location on the deposition rate of aerosolized Staphylococcus aureus in a 32?m3 chamber. Increasing the ventilation rate from 3 to 6 ACH results in a reduction of microbial load in the air and on surfaces by 45%?±?10% and 44%?±?32%, respectively. The deposition rate onto internal surfaces ?d was calculated as 1.38?±?0.48?h?1. Samples of airborne and surface microorganisms taken closer to the ventilation supply showed a lower concentration than close to the extract. The findings support the importance of controlling the ventilation and the environmental parameters to mitigate both air and surface infection risks in the hospital environment.