Source avec lien : American Journal of Infection Control, 50(8). 10.1016/j.ajic.2022.03.015
Nous avons examiné la décontamination de la variante Beta du SRAS-CoV-2 sur des masques chirurgicaux et des masques réutilisables Revolution-Zero Environmentally Sustainable (RZES) en utilisant ces colorants photoactivés. Nous avons prétraité les masques chirurgicaux avec 2 concentrations de MB, 2 concentrations de RB et 2 combinaisons de MB et de RB. Nous avons également testé 7 concentrations de MB sur des masques RZES.
Background Methylene blue (MB) and riboflavin (RB) are light-activated dyes with demonstrated antimicrobial activity. They require no specialized equipment, making them attractive for widespread use. Due to COVID-19-related worldwide shortages of surgical masks, simple, safe, and effective decontamination methods for reusing masks have become desirable in clinical and public settings. Material and methods We examined the decontamination of SARS-CoV-2 Beta variant on surgical masks and Revolution-Zero Environmentally Sustainable (RZES) reusable masks using these photoactivated dyes. We pre-treated surgical masks with 2 MB concentrations, 2 RB concentrations, and 2 combinations of MB and RB. We also tested 7 MB concentrations on RZES masks. Results Photoactivated MB consistently inactivated SARS-CoV-2 at >99.9% for concentrations of 2.6 µM or higher within 30 min on RZES masks and 5 µM or higher within 5 min on disposable surgical masks. RB alone showed a lower, yet still significant inactivation (∼93-99%) in these conditions. Discussion MB represents a cost-effective, rapid, and widely deployable decontamination method for SARS-CoV-2. The simplicity of MB formulation makes it ideal for mask pre-treatment in low-resource settings. Conclusions The results demonstrate that MB effectively decontaminates SARS-CoV-2 at concentrations above 5 µM on surgical masks and above 10 µM on RZES masks.